Nuclear Structure as Revealed by Electric Excitation

Nuclear pore complex structure and dynamics revealed by cryoelectron tomography.

Nuclear pore complexes (NPCs) are gateways for nucleocytoplasmic exchange. To analyze their structure in a close-to-life state, we studied transport-active, intact nuclei from Dictyostelium discoideum by means of cryoelectron tomography. Subvolumes of the tomograms containing individual NPCs were extracted in silico and subjected to three-dimensional classification and averaging, whereby distin...

Electric Excitation of Nerve

Surface structure of Azotobactervinelandii cysts as revealed by freeze-cleaving.

Micrographs of freeze-cleaved Azotobacter vinelandii cysts reveal that the surface is composed of several overlapping layers. This observation is consistent with the previously proposed structure of the outer cyst coat.

Immunoglobulin structure and function as revealed by electron microscopy.

Electron-microscopic (EM) analysis preceded crystallographic analysis [1,2] of Igs by over a decade and was for a time the only direct way of analyzing their 3-D molecular structure. Once the X-ray structures were deduced, the role of EM gradually shifted from gross structural analysis to the addressing of more sophisticated structural and functional questions. EM remains a vital adjunct to the...

Convective Structure of Hurricanes as Revealed by Lightning Locations

Cloud-to-ground lightning flash locations were examined for nine Atlantic basin hurricanes using data from the National Lightning Detection Network. A common radial distribution in ground flash density was evident: a weak maximum in the eyewall region, a clear minimum 80–100 km outside the eyewall, and a strong maximum in the vicinity of outer rainbands (210–290-km radius). These results are co...